Semen testing and freezing is charged with a total sum which is relative to the total number of collected samples – there is a discount in case more than one samples are collected.

The cryopreservation costs are determined on a monthly basis and cover all cryopreserved samples.

Thawing and transport costs are also charged to the patient.

When you are ready to use your cryopreserved semen for an assisted reproductive method, you must notify the Sperm Bank as soon as possible, preferably 1-2 weeks before the scheduled attempt.

The semen sample can be transferred to any doctor or fertility clinic you specify on a written special consent form.

During thawing, some of the frozen spermatozoa do not survive. The recovery of functional spermatozoa that survive the freeze-thawing cycle depends on the endogenous endurance of each semen sample and cannot be predicted with certainty.

Every semen sample is carefully identified using a special coding system and labeling, which ensures the precise traceability and confidentiality of the data. Each sample is labeled with the patient’s name, a unique code and the date of freezing.

A small aliquot of every semen sample is examined with regard to the basic sperm parameters before freezing, i.e.:

• Semen volume
• Sperm motility
• Motility index

The rest of the sample is diluted with a special cryoprotectant medium, which protects the biological properties of the spermatozoa during the freezing process.

The sample is gradually put in special freezing containers (straws or paillettes) at several aliquots of low volume (0.5 ml). The total number of straws depends on the total volume of the semen sample.

The freezing process starts directly after the straw filling and is performed using a semi-automated system which permits the gradual temperature decrease from room temperature to ultra low levels (< 0 °C). This step protects the spermatozoa from the damage that an abrupt temperature fall would cause.

At the end of the freezing cycle, the samples are submerged in liquid nitrogen and then placed in special long term cryopreservation tanks.

Semen may be preserved at a final temperature of -196 °C indefinitely. CRYOGONIA Sperm Bank offers semen cryopreservation for long time periods, following the patient’s consent and its renewal at regular intervals.

Semen samples may be collected in a special, comfortable room located in the Sperm Bank. In case the patient is in the hospital, it is also possible that an authorized person can transfer the samples with specific appointment.

You are advised to arrange an appointment before your visit to the Sperm Bank. Specific instructions regarding the sample’s collection and transfer are provided by the personnel of CRYOGONIA during the phone communication.