Usually, it is advised to cryopreserve multiple samples, but if the time frame is limited, keeping even one sample is worth the effort.
With the application of modern in vitro fertilization techniques, it is possible to create an embryo by the injection of one live spermatozoon in an oocyte. Sperm cryopreservation is therefore a justifiable choice, even if semen quality is relatively low.
Generally, preserving more semen samples allows the performance of more reproductive cycles.
For every intended pregnancy, it is recommended to collect 3-6 semen samples, depending on sperm quality.