Laboratory testing of prospective donors includes multiple seminological and haematological tests:
- Conventional parameters (semen volume, sperm count, motility, morphology, vitality)
- Functional parameters (acrosome integrity test, sperm DNA integrity test, semen redox potential test, etc.)
- Recovery test and longevity study post thaw
- Complete microbiological screening
- Blood type (ABO) / Rh
- Karyotypic profile
- Cystic fibrosis (includes 90-99% of detectable pathological mutations in the Greek population) (molecular testing with NGS since June 2017)
- Mediterranean syndromes (type α, β, δβ and sickle cell anaemia)
- Spinal muscular atrophy (SMA) (since June 2015)
- Sexually Transmissible Diseases (HIV I / II, HbsAg, anti-HCV, anti-HTLV, CMV, VDRL)
Only the candidates whose semen quality exceeds the reference values of internationally recognized methodologies are selected.
Due to the seasonal variations observed in semen, it is possible that a candidate donor is asked to deliver 2 or 3 control samples, in order to certify whether his semen quality meets the selection criteria.
There is also a possibility that neat semen of good quality might not be adequately recovered post thaw. Since sperm donation requires only frozen semen, the selection obviously includes only samples that produce good recovery of motile spermatozoa after thawing.