Sperm cryopreservation – a laboratory method with long history

12ο Πανελλήνιο Συνέδριο Ανδρολογίας (4-6 Ιουνίου 2021)

The technology behind the cryopreservation of reproductive cells and tissues is based on scientific principles that were evolved during the past 50 years.

The use of liquid nitrogen in sperm cryopreservation at -196oC was introduced during the 1960s, followed by reports of success in assisted reproductive methods, pregnancies and births.

The first successful pregnancy by use of frozen sperm was achieved by Prof. Sherman in 1953.

The first successful birth of a normal child by use of frozen sperm was achieved in1973.

The first organized Sperm Banks were created in the early 1970s (1972).

The longest semen cryopreservation duration hitherto before use with successful birth is 21 years.